测定DNA含量用什么方法
DNA含量的测定是生物学和分子生物学研究中的重要环节，它对于基因表达、细胞周期、染色体异常分析等领域的研究至关重要。以下是一些常用的测定DNA含量的方法：
1. 分光光度法
分光光度法是最常用的DNA含量测定方法之一。这种方法基于DNA在特定波长下的光吸收特性。通常使用260nm和280nm的波长来测定DNA和蛋白质的浓度。
信息来源：NCBI的综述文章《Quantitation of DNA in solution》提供了详细的信息。[链接](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2973351/)
2.琼脂糖凝胶电泳
琼脂糖凝胶电泳是一种通过电泳分离DNA片段并定量不同大小DNA的方法。通过比较已知浓度的DNA标准品的迁移率，可以估算样品中DNA的含量。
信息来源：BioRad的指南《Agarose Gel Electrophoresis of DNA》提供了琼脂糖凝胶电泳的详细操作步骤。[链接](https://www.biorad.com/webroot/web/pdf/lsr/pdf/lsr_5210_5220_5230_5240_5250_5260_5270_5280_5290_5300_5310_5320_5330_5340_5350_5360_5370_5380_5390_5400_5410_5420_5430_5440_5450_5460_5470_5480_5490_5500_5510_5520_5530_5540_5550_5560_5570_5580_5590_5600_5610_5620_5630_5640_5650_5660_5670_5680_5690_5700_5710_5720_5730_5740_5750_5760_5770_5780_5790_5800_5810_5820_5830_5840_5850_5860_5870_5880_5890_5900_5910_5920_5930_5940_5950_5960_5970_5980_5990_6000_6010_6020_6030_6040_6050_6060_6070_6080_6090_6100_6110_6120_6130_6140_6150_6160_6170_6180_6190_6200_6210_6220_6230_6240_6250_6260_6270_6280_6290_6300_6310_6320_6330_6340_6350_6360_6370_6380_6390_6400_6410_6420_6430_6440_6450_6460_6470_6480_6490_6500_6510_6520_6530_6540_6550_6560_6570_6580_6590_6600_6610_6620_6630_6640_6650_6660_6670_6680_6690_6700_6710_6720_6730_6740_6750_6760_6770_6780_6790_6800_6810_6820_6830_6840_6850_6860_6870_6880_6890_6900_6910_6920_6930_6940_6950_6960_6970_6980_6990_7000_7010_7020_7030_7040_7050_7060_7070_7080_7090_7100_7110_7120_7130_7140_7150_7160_7170
